Expert researchers and physicians not only describe classic and contemporary laboratory methods for studying tissue repair using a broad range of wound healing models, but also provide sensitive assays for the assessment of tissue healing. The systems detailed include human, animal, in vitro, in vivo, and impaired healing models, as well as models in which underlying systemic and genetic conditions influence the healing process. These powerful models illustrate the biochemical, molecular, and surgical techniques designed for the analysis and manipulation of the healing wound, and provide step-by-step instructions to ensure robust results.