This book addresses recent developments in enzymes and HPLC methods, along with their use in various laboratory applications. This includes the application of HPLC and enzymes to more disciplines; advances in separation techniques and instrumentation that have increased HPLC capability; and the discovery of new enzymes and resulting new methods of analysis. It offers the same practical approach found in the previous editions, incorporates a wealth of new information into existing chapters, and adds new chapters to deal with new applications, including capillary electrophoresis, forensic chemistry, microdialysis, and the polymerase chain reaction.