The second edition of Adhesion Protein Protocols combines traditional
techniques with cutting-edge and novel techniques that can be adapted
easily to different molecules and cell types. The topics discussed in
this updated second edition include novel techniques for studying
cell-cell adhesion, neutrophil chemotaxis, in vitro assays used to
study leukocyte migration through monolayers of cultured endothelial
cells, and novel techniques to purify pseudopodia from migratory cells.
This book also discusses the study of cell-matrix interaction, RNA
interference, fluorescence recovery after photobleaching, actin
purification, the application of microarray techniques, and the role of
adhesion proteins in the study of proteomics.
The protocols discussed in this volume are suitable for both novice and
expert scientists, who will gain further insight into the complex and
incompletely understood processes involved in cellular adhesion.
